Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (29): 5401-5406.doi: 10.3969/j.issn. 2095-4344.2012.29. 017

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Effect of the combination of glial cell line-derived neurotrophic factor, NogoA and chondroitin sulfate ABC microspheres on promoting the neural regeneration of rat’s injured spinal cord

Zhang Yu1,2, Song Yue-ming1   

  1. 1Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China;
    2Department of Orthopedics, Chengdu First People’s Hospital, Chengdu 610021, Sichuan Province, China
  • Received:2012-03-15 Revised:2012-04-20 Online:2012-07-15 Published:2012-07-15
  • Contact: Song Yue-ming, Professor, Doctoral supervisor, Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China yuxli@cisco.com
  • About author:Zhang Yu☆, Doctor, Attending physician, Department of Orthopedics, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China; Department of Orthopedics, Chengdu First People’s Hospital, Chengdu 610021, Sichuan Province, China yuxli@cisco.com

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Abstract:

BACKGROUND: Previous studies have shown that glial cell line-derived neurotrophic factor (GDNF) exhibits good effect on the regeneration of the injured spinal cord.
OBJECTIVE: To investigate the effect of the combination of the injured chondroitin sulfate ABC (ChABC), GDNF and NogoA microspheres on the regeneration and functional recovery of the injured spinal cord in rats.
METHODS: Totally 64 adult SD female rats were randomly divided into eight groups: normal control group, sham operation group (only laminectomy), model group, GDNF group, ChABC microspheres group, GDNF microspheres group, NogoA microspheres group, ChABC+GDNF+NogoA microspheres group. T10 total transected spinal cord models were established in all groups except for normal control and sham operation groups, and the broken ends of the injured spinal cord were injected a half of liquid medicine, and then the back of the broken ends were covered by the other half of liquid medicine which was absorbed by latin sponge.
RESULTS AND CONCLUSION: ①Basso, Beattie and Bresnahan (BBB) Locomotor Rating scores: ChABC+GDNF +NogoA microspheres group was higher than the model, GDNF, GDNF microspheres, ChABC and NogoA microspheres groups at weeks 5, 6, 7, 8, 9 and 10 after treatment (P < 0.05). ②Cortical somatosensory evoked potential (CSEP) at week 10 after treatment: no detection was found in the sham operation , model and GDNF groups. CSEP in main reaction latency of the ChABC+GDNF+NogoA microspheres group was lower than that of other groups (P < 0.05), but higher than that of the normal control group (P < 0.05). The peak value of the main reaction peak in the ChABC+GDNF+NogoA microspheres group was higher than that in other groups (P < 0.05), but lower than that in the normal control group (P < 0.05). These findings suggest that the combination of GDNF, NogoA and ChABC microspheres can promote the regeneration, functional recovery and nerve conduction of the spinal cord in rats, which is superior to that of pure GDNF microspheres.

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